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  • Bruno Vellutini 19:27 on 2013/03/07 Permalink
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    Fluorescent in situ of Terebratalia engrailed 


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    Mounted a slide with Terebratalia embryos from the first fluorescent/double in situ I tried for the segmentation genes and observed under the confocal. Mounting media had DAPI (magenta) and en is green. Although there is background it is possible to distinguish the real signal.

    Here are some max-projections to illustrate:

    MAX_Ttra En_DNP + DAPI.lif - Series031 MAX_Ttra En_DNP + DAPI.lif - Series028 MAX_Ttra En_DNP + DAPI.lif - Series031

     
  • Bruno Vellutini 14:25 on 2013/03/07 Permalink
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    Just had a look at the Wnts with the new Trinity assembly. In summary:

    • Sequences from both assemblies match up nicely and the only difference is in the length of contigs.
    • Some genes remained the same: Wnt4, Wnt5, Wnt8.
    • Two were longer in one direction, but shorter in the other end: Wnt7, Wnt10.
    • Some were considerably or slightly longer in one or both directions: Wnt1, Wnt9, Wnt16, WntA.
    • Two were slightly shorter than Dunn’s assembly: Wnt6, Wnt11.
    • Wnt2 was resolved into a single contig (previously split in two short fragments).

    Tt_wnt2

    Quality seems to be equivalent (there were no mismatched portions) and there are only a few base pairs differing, but Trinity gave longer contigs; and was able to assemble Wnt2. Files are in the Dropbox.

    Fritzen is conducting a couple of tests for comparing both assemblies globally..

     
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