Starting a Terebratalia RNAseq CLC assembly with the following parameters:
Automatic word size = ON Automatic bubble size = ON Minimum contig length = 170 Auto-detect paired distances = ON Perform scaffolding = OFF Map reads back = ON mismatch = 2 insertion = 3 deletion = 3 length = 0.5 similarity = 0.8 Colorspace alignment = ON Global alignment = OFF Update contigs = ON Create list of un-mapped = OFF Create report = ON Result handling = Save Make log = ON
Started a PCR with Terebratalia wnta, wnt2; and Meara ago a, ago b, ago c, bru a, bru b, mago, mex3, orb2.
Put colonies to grow.
| Colony | T7 |
| Ago a c1 | BV235 |
| Ago a c2 | BV236 |
| Ago a c3 | BV237 |
| Ago b c1 | BV238 |
| Ago b c2 | BV239 |
| Ago b c4 | BV240 |
| Bru a c1 | BV241 |
| Bru a c2 | BV242 |
| Bru a c3 | BV243 |
| Mago c1 | BV244 |
| Mago c2 | BV245 |
| Mago c3 | BV246 |
| Orb2 c1 | BV247 |
After last Lineus collection I rearranged the worms collected a year ago to a small glass bowl. Some time in the last week they laid two egg cases which I found today. I transferred the two egg cases to a small glass bowl with clean sea water.
So apparently we can keep these worms in the facility with irregular and scarce food supply and they still manage to lay eggs.
Recently collected worms did not lay anything yet.
Starting a Isodiametra RNAseq CLC assembly with the following parameters:
Automatic word size = ON Automatic bubble size = ON Minimum contig length = 170 Auto-detect paired distances = ON Perform scaffolding = OFF Map reads back = ON mismatch = 2 insertion = 3 deletion = 3 length = 0.5 similarity = 0.8 Colorspace alignment = ON Global alignment = OFF Update contigs = ON Create list of un-mapped = OFF Create report = ON Result handling = Save Make log = ON
It started at 16h and finished 01h00. ~9h running with 24 cores.
Starting a Xenoturbella RNAseq CLC assembly with the following parameters:
Automatic word size = ON Automatic bubble size = ON Minimum contig length = 170 Auto-detect paired distances = ON Perform scaffolding = OFF Map reads back = ON mismatch = 2 insertion = 3 deletion = 3 length = 0.5 similarity = 0.8 Colorspace alignment = ON Global alignment = OFF Update contigs = ON Create list of un-mapped = OFF Create report = ON Result handling = Save Make log = ON
It started at 13h30 and finished 23h00. ~12h running with 24 cores.
Took some lateral views of en+wnt1 and a few scans of en+wnt5. The latter is quite noisy for wnt5 probe unfortunately…
Began confocalling and they look good :)
Sorted the collected nemerteans. It looks like there are two main morphotypes that could be Lineus. A more reddish as we use to call Lineus ruber and a more greenish/yellowish. The latter can have a gradient from red to green in the A/P axis. Also there are thin ones that might be different or juvenile stages.
A different morphotype is whiteish with 2 distinct pink brain areas. Finally there is a similar to Lineus above, but in dark green.
Set a transcription reaction to synthesize more DNP probe for Ttra engrailed. Making 4 tubes and initiated precipitation at 18h.
Collected a bunch of nemertines with Henrike and Aina at Aina’s beach.
