Some cloning of Lineus and Priapulus 

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Cloning of Pc gmcl, Lr caudal, Lr goosecoid, Lr foxq2, Lr fgf 8/17/18. Running the PCR with 60 °C for annealing, adjusted to primers Tm.


Running gel:

I cut caudal and goosecoid bands. Extracted, ligated/transformed.


Colony PCR:


Minipreps for cloning:

Colony T7
cdx 6 BV202
cdx 7 BV203
cdx 8 BV204
gsc 6 BV205
gsc 7 BV206
gsc 8 BV207