RNA Extraction of Priapulus staged samples 


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In order to estimate the amount of embryos needed for doing the RNA-Seq of specific stages I am extracting RNA from previousvly fixed samples. I extracted total RNA with Trizol, following Seaver protocol. Had to add equal volume of PBS to RNAlater to make embryos sink.

Stage Embryos Volume (µL) ng/µL 260/280 260/230 RNA (ng)
Gastrula ~650 20 80.22 1.55 1.16 1604.4
Introvertula ~1070 20 88.89 1.78 1.04 1777.8
2nd larva ~570 20 60.56 1.72 0.37 1211.2