Lineus ruber first cloning 


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Trying to clone 6 genes: six 3/6, caudal, goosecoid, foxa, foxq2, and fgf8/17/18. PCR was not so successful, only foxa and six3/6 worked:

30/10/12

Set ligation.

31/10/12

Transformed and plated.

01/11/12

Colony PCR for both genes:

03/11/12

Minipreps.

Colony T7
foxa 4 BV196
foxa 5 BV197
foxa 6 BV198
six3/6 6 BV199
six3/6 7 BV200
six3/6 8 BV201