Membranipora in situ Syt1a and Nanos


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In situ with Membranipora for testing the newly fixed material with glutaraldehyde. I’m using 4 conditions and 2 genes:

ProtK Time Gene Gene
0.5x 10 min Syt1a Nanos
0.5x 20 min Syt1a Nanos
1x 20 min Syt1a Nanos
2x 20 min Syt1a Nanos

31/10/12

Developing the in situ. Wells with nanos already showing the background all around with no difference from treatments. Synaptotagmin looks more promising, no background still. First well seems to have a slight purpleish regions, but others are plain white. Exchanged the AP twice and let it overnight in cold room.

01/11/12

Nanos is completely background. On syt1a I think there is signal in the last two treatments (1x and 2x). The lighter treatments seem to have some background. I exchanged the AP twice and will stop the reaction today.

02/11/12

It seems that synaptotagmin works.