ProteinaseK test for Membranipora


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Tested Membranipora to see how do I have to treat them in ProteinaseK during the in situ. I put 5 larvae in 0.005 mg/mL (half-concentrated) and waited 1h checking every 5 minutes. Nothing changed. I added +2.5 ┬ÁL of ProtK (up to 0.01 mg/mL) and waited 30 min more and nothing changed. So I left the embryos overnight and in the following morning only the shell was left, soft tissues were dissolved.