ProteinaseK test for Membranipora 

Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/ on line 77

Tested Membranipora to see how do I have to treat them in ProteinaseK during the in situ. I put 5 larvae in 0.005 mg/mL (half-concentrated) and waited 1h checking every 5 minutes. Nothing changed. I added +2.5 ┬ÁL of ProtK (up to 0.01 mg/mL) and waited 30 min more and nothing changed. So I left the embryos overnight and in the following morning only the shell was left, soft tissues were dissolved.