Membranipora in situ with new material


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Starting an in situ with the newly fixed material of Membranipora. I took 200 µL from X, X, X, X days and mixed. Since samples were fixed with 3.7% PFA + 0.2% Gluta I am testing the time in proteinase K for 2 genes, Synaptotagmin and Nanos (a strong one and a known one), with 30s and 1:30s.

30s 1m30s
Mm gene Syt1a Syt1a
Mm gene Nanos Nanos

17/10/12

Started developing at 11h30. By night a weak background began to appear in the shell.

18/10/12

Change AP at 10h. Background became stronger and apparently no real signal neither in syt or nos. Some larvae in the syt wells do not have much background. Crystals appeared in the afternoon… Kept developing at 4 °C.

21/10/12

Stopped developing.

24/10/12

Checked under microscope… only background it seems.