In situ of Terebratalia wnt genes

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Starting in situ for wnt genes and segmentation genes:

wnt1 wnt9 gli
wnt5 wnt10 smo
wnt6 wnt16 nk6

All genes took 2 days to developed, except for wnt9, wnt10 and smo. I let them all in AP -MgCl2 for the weekend and resumed developing on Monday morning. I exchanged the 3 wells for new AP substrate and let overnight at 4 °C.