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  • Bruno Vellutini 16:19 on 2012/09/14 Permalink
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    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Aina brought new Membranipora today. I’ll use them next week for inhibitor experiments.

    They were not good… dirty and very few embryos released.

     
  • Bruno Vellutini 15:54 on 2012/09/14 Permalink
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    In situ of Terebratalia wnt genes 


    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Starting in situ for wnt genes and segmentation genes:

    wnt1 wnt9 gli
    wnt5 wnt10 smo
    wnt6 wnt16 nk6

    All genes took 2 days to developed, except for wnt9, wnt10 and smo. I let them all in AP -MgCl2 for the weekend and resumed developing on Monday morning. I exchanged the 3 wells for new AP substrate and let overnight at 4 °C.

     
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