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  • Bruno Vellutini 22:17 on 2012/09/24 Permalink
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    Membranipora larvae with 34 d fed from the beginning are larger than starved larvae. One larva had an invagination at the posterior part of the corona which is the sole indication of a presumptive internal sac. Nothing seems to be growing where nanos is expressed.

     
  • Bruno Vellutini 16:19 on 2012/09/14 Permalink
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    Aina brought new Membranipora today. I’ll use them next week for inhibitor experiments.

    They were not good… dirty and very few embryos released.

     
  • Bruno Vellutini 15:54 on 2012/09/14 Permalink
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    In situ of Terebratalia wnt genes 


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    Starting in situ for wnt genes and segmentation genes:

    wnt1 wnt9 gli
    wnt5 wnt10 smo
    wnt6 wnt16 nk6

    All genes took 2 days to developed, except for wnt9, wnt10 and smo. I let them all in AP -MgCl2 for the weekend and resumed developing on Monday morning. I exchanged the 3 wells for new AP substrate and let overnight at 4 °C.

     
  • Bruno Vellutini 14:35 on 2012/09/12 Permalink
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    Pc gmcl and Pc piwia 


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    On 05/09/12 I did a PCR for both. Only today I ran the gel and there was no band. Just setup a new reaction with lower annealing temperature (63 °C).

     
  • Bruno Vellutini 18:49 on 2012/09/04 Permalink
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    Found the 4D recording scope with a lot of condensation in the objective and both sides of the slide. The optics were terribly affected by the condensation between the slide and the condenser. Embryo failed…

     
  • Bruno Vellutini 22:43 on 2012/09/03 Permalink
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    Today Andi, Aina, and me went on the boat to collect Novocrania anomala and Meara stichopi.

    N. anomala was collected by dredging rocky bottom and looking for specimens attached to rocks and shells. Rocks were kept in a large box with sea water and transferred to S4 tanks with flowing sea water at Sars.

    M. stichopi was also collected by dredging, but on mud bottom. Around 20 specimens were captured in a new spot: 60°14.946 N, 05°08.481 O

     
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