Membranipora larvae with 34 d fed from the beginning are larger than starved larvae. One larva had an invagination at the posterior part of the corona which is the sole indication of a presumptive internal sac. Nothing seems to be growing where nanos is expressed.
Aina brought new Membranipora today. I’ll use them next week for inhibitor experiments.
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They were not good… dirty and very few embryos released.
Starting in situ for wnt genes and segmentation genes:
| wnt1 | wnt9 | gli |
| wnt5 | wnt10 | smo |
| wnt6 | wnt16 | nk6 |
All genes took 2 days to developed, except for wnt9, wnt10 and smo. I let them all in AP -MgCl2 for the weekend and resumed developing on Monday morning. I exchanged the 3 wells for new AP substrate and let overnight at 4 °C.
On 05/09/12 I did a PCR for both. Only today I ran the gel and there was no band. Just setup a new reaction with lower annealing temperature (63 °C).
Found the 4D recording scope with a lot of condensation in the objective and both sides of the slide. The optics were terribly affected by the condensation between the slide and the condenser. Embryo failed…
Today Andi, Aina, and me went on the boat to collect Novocrania anomala and Meara stichopi.
N. anomala was collected by dredging rocky bottom and looking for specimens attached to rocks and shells. Rocks were kept in a large box with sea water and transferred to S4 tanks with flowing sea water at Sars.
M. stichopi was also collected by dredging, but on mud bottom. Around 20 specimens were captured in a new spot: 60°14.946 N, 05°08.481 O
