Membranipora larvae with 34 d fed from the beginning are larger than starved larvae. One larva had an invagination at the posterior part of the corona which is the sole indication of a presumptive internal sac. Nothing seems to be growing where nanos is expressed.
Updates from September, 2012 Toggle Comment Threads | Keyboard Shortcuts
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Bruno Vellutini
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Bruno Vellutini
Aina brought new Membranipora today. I’ll use them next week for inhibitor experiments.
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They were not good… dirty and very few embryos released.
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Bruno Vellutini
In situ of Terebratalia wnt genes
Starting in situ for wnt genes and segmentation genes:
wnt1 wnt9 gli wnt5 wnt10 smo wnt6 wnt16 nk6 All genes took 2 days to developed, except for wnt9, wnt10 and smo. I let them all in AP -MgCl2 for the weekend and resumed developing on Monday morning. I exchanged the 3 wells for new AP substrate and let overnight at 4 °C.
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Bruno Vellutini
Pc gmcl and Pc piwia
On 05/09/12 I did a PCR for both. Only today I ran the gel and there was no band. Just setup a new reaction with lower annealing temperature (63 °C).
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Bruno Vellutini
Found the 4D recording scope with a lot of condensation in the objective and both sides of the slide. The optics were terribly affected by the condensation between the slide and the condenser. Embryo failed…
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Bruno Vellutini
Today Andi, Aina, and me went on the boat to collect Novocrania anomala and Meara stichopi.
N. anomala was collected by dredging rocky bottom and looking for specimens attached to rocks and shells. Rocks were kept in a large box with sea water and transferred to S4 tanks with flowing sea water at Sars.
M. stichopi was also collected by dredging, but on mud bottom. Around 20 specimens were captured in a new spot: 60°14.946 N, 05°08.481 O
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