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  • Spawning activated with EDTA at 12:30. Fixed for in situ and confocal at: 0-2h, 2-4h, 4-6h.
  • Spawning activated at 20:30 for fixing later stages.
  • Found out that pen-strep water is essential for 4D microscopy. Maybe it is a good idea to use it in normal cultures.
  • Set a recording that seems to be working.