Updates from August, 2012 Toggle Comment Threads | Keyboard Shortcuts

  • Bruno Vellutini 18:13 on 2012/08/06 Permalink
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    Tried to feed early cyphonautes (8 day old) with Rhodomonas. One larva captured the particle (into the atrium), but it remained stuck in the mouth region. Maybe it is not ready yet? After a couple of minutes the larva ejected the algae.

     
  • Bruno Vellutini 14:30 on 2012/08/06 Permalink
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    Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

    Yesterday spawning seems to be a bit more successful. I would say that 50-60% were at advanced cleavage stages (~64cell) by this morning. The only difference from previous spawning was that I added 50 ┬ÁL of 0.5M EDTA to the bowl (making it correctly 0.1mM, since the bowl has about 250 mL) and did the first washes relatively gently, without resuspending the embryos.

    Today I’m also trying collecting the embryos directly from the bowl (as I was doing before) and making only a water change to 0.1 mM EDTA solution, also super gently, using a gelatin coated dish. The reason for this is that I did not see a significant increase in the embryo activation by letting the EDTA period in the bowl. Maybe doing this in the dish and doing it gently might work well.

    Well, after 2h of EDTA in the cold room the eggs did not look so much more activated than the usual. There were some 2 cell stages, but not very expressive. Have to check tomorrow.

     
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