PCR mostly stem/germ cell genes of Terebratalia


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Setting up a pcr with gradient (63 +- 3 °C) at 12h:

Gene Primers Tm mean
Mm Six3/6 64.10
Tt Dicer1b 61.60
Tt Runx 63.15
Tt Mael 65.15
Tt Piwia 65.55
Tt Tudor1 61.95
Tt Ago 62.95
Tt PL10 63.10
Tt Pum 65.35

Extraction and ligation overnight at 4°C, except for MAEL.

24/07/12

Transformation and plating of colonies.

25/07/12

PL10 and Pumilio had very few colonies on the plate (insert numbers) and together with Piwia they did not produce any positive colony.

27/07/12

Put positive colonies to grow for minipreps.

BV162 Terebratalia transversa Dicer1b
BV163 Terebratalia transversa Dicer1b
BV164 Terebratalia transversa Runx2
BV165 Terebratalia transversa Runx2
BV166 Terebratalia transversa Runx2
BV167 Terebratalia transversa Tudor1
BV168 Terebratalia transversa Argonaute
BV169 Terebratalia transversa Argonaute
BV170 Terebratalia transversa Argonaute
BV171 Terebratalia transversa NK6
BV172 Terebratalia transversa NK6
BV173 Terebratalia transversa NK6
BV174 Terebratalia transversa Wnt7
BV175 Terebratalia transversa Wnt7
BV176 Terebratalia transversa Wnt7