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We collected a jellyfish for muscle Phallacidin staining for Andi’s News & Views article. I cut small pieces of the marginal region of the umbrella and followed the standard fixation protocol for confocal stainings. I also cut larger portions of the same region and fixed in 4% PFA overnight at 4 °C.

30/05/12

Washed the larger portions in PBS+Triton-X during the whole day and transferred to PBS at 4 °C. I stained one well with Phallacidin and looked in the fluorescent lamp to find the muscle layer. It is an opaque sheath of tissue below the mesoglea. The staining worked.

31/05/12

Dissected the muscle layer better and mounted the slide. The tissue is well spread and staining looks ok. Confocal tomorrow.

01/06/12

Confocal time.