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We collected a jellyfish for muscle Phallacidin staining for Andi’s News & Views article. I cut small pieces of the marginal region of the umbrella and followed the standard fixation protocol for confocal stainings. I also cut larger portions of the same region and fixed in 4% PFA overnight at 4 °C.


Washed the larger portions in PBS+Triton-X during the whole day and transferred to PBS at 4 °C. I stained one well with Phallacidin and looked in the fluorescent lamp to find the muscle layer. It is an opaque sheath of tissue below the mesoglea. The staining worked.


Dissected the muscle layer better and mounted the slide. The tissue is well spread and staining looks ok. Confocal tomorrow.


Confocal time.