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Fixation of 7 d old L. ruber for confocal and in situ. Bissected the halves of egg masses from previous fixation and removed the outer case. Instead of treating with cystein I simply used the large plastic pipet to dissociate the eggs from the jelly. It worked quite well, even though the jelly would stick in the pipet.

Eggs were release from the bundle with needles and fixed with the standard protocol for in situ and confocal.