Cloning Tt Piwia, hh, Smo2, Nk2.2, NK5 and Mm Runx


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Half of the plates from this batch that I had not sequenced yet. Possibly contaminated with Wnts, so I’ll need to check the bands carefully.

04/05/12

Plasmid extraction and minipreps are ready for sequencing PCR.

BV125 Terebratalia transversa Piwia
BV126 Terebratalia transversa Piwia
BV127 Terebratalia transversa Piwia
BV128 Terebratalia transversa Hedgehog
BV129 Terebratalia transversa Hedgehog
BV130 Terebratalia transversa Hedgehog
BV131 Terebratalia transversa Smoothened2
BV132 Terebratalia transversa Smoothened2
BV133 Terebratalia transversa Smoothened2
BV134 Terebratalia transversa NK2.2
BV135 Terebratalia transversa NK2.2
BV136 Terebratalia transversa NK2.2
BV137 Terebratalia transversa NK5
BV138 Terebratalia transversa NK5
BV139 Terebratalia transversa NK5
BV140 Membranipora membranacea Runx
BV141 Membranipora membranacea Runx
BV142 Membranipora membranacea Runx

05/05/12

Sequencing PCR executed and samples sent to the sequencing facility.

08/05/12

Got the sequences back and they all look good and match the original sequence, except for TtPiwia, which hits Wnt1 and Wnt16. Since it is the second time I try to clone it I’ll probably re-do this gene from scratch.