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  • Bruno Vellutini 19:00 on 2012/05/03 Permalink  


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    Observed that the longer and thinner nemerteans triaged on Tuesday are fragmenting. I’ll keep the fragments to see if they will regenerate.

     
  • Bruno Vellutini 12:59 on 2012/05/03 Permalink
    Tags: , , , , , , ,   

    Cloning Tt Piwia, hh, Smo2, Nk2.2, NK5 and Mm Runx 


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    Half of the plates from this batch that I had not sequenced yet. Possibly contaminated with Wnts, so I’ll need to check the bands carefully.

    04/05/12

    Plasmid extraction and minipreps are ready for sequencing PCR.

    BV125 Terebratalia transversa Piwia
    BV126 Terebratalia transversa Piwia
    BV127 Terebratalia transversa Piwia
    BV128 Terebratalia transversa Hedgehog
    BV129 Terebratalia transversa Hedgehog
    BV130 Terebratalia transversa Hedgehog
    BV131 Terebratalia transversa Smoothened2
    BV132 Terebratalia transversa Smoothened2
    BV133 Terebratalia transversa Smoothened2
    BV134 Terebratalia transversa NK2.2
    BV135 Terebratalia transversa NK2.2
    BV136 Terebratalia transversa NK2.2
    BV137 Terebratalia transversa NK5
    BV138 Terebratalia transversa NK5
    BV139 Terebratalia transversa NK5
    BV140 Membranipora membranacea Runx
    BV141 Membranipora membranacea Runx
    BV142 Membranipora membranacea Runx

    05/05/12

    Sequencing PCR executed and samples sent to the sequencing facility.

    08/05/12

    Got the sequences back and they all look good and match the original sequence, except for TtPiwia, which hits Wnt1 and Wnt16. Since it is the second time I try to clone it I’ll probably re-do this gene from scratch.

     
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