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  • Bruno Vellutini 15:00 on 2012/05/01 Permalink
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    Triaged nemerteans collected by Aina. Found 3 types, Lineus ruber darker color and thicker worms; a thinner, clearer (but still reddish) and longer worm; a yellowish shorter worm with pink eyes. Had a quick look in the Norwegian fauna book, but did not find the other two. The worms were separated in 3 bowls.

     
  • Bruno Vellutini 13:00 on 2012/05/01 Permalink
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    Captured confocal images of the L. ruber fixed with and without cysteine treatment in samples not treated with Murray’s clear. Stained with Phallacidin and Propidium Iodide. It seems that the juvenile tissues are well preserved and with no disruptions, although I could see few epithelial cells that apparently popped off.

    Using the 20x objective I could acquire whole body images and also a closeup of the anterior end in 2 individuals. Early stages (10d) seem to be only rubbish, strange blastomeres whose nuclei were not stained. Seem to be degenerating. Probably unfertilized eggs or interrupted embryos, although I still need to analyze the images properly.

     
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