Debugging contamination of Wnts

Four of the sequences from 2012-04-25_0446 were contamination of Terebratalia Wnts in my samples. Wnts had already appeared in the sequences of Henrike, but the source was not identified. To identify if the contamination is in the sequencing reaction or the actual Wnts are being cloned Chema suggested to run a PCR with the miniprep using the gene specific primers. I also included the specific Terebratalia primers to see if there is any amplification.

ID Gene Actual sequence
BV93 Mm Piwi1 Tt Wnt16
BV95 Mm Piwi1 Tt Wnt10
BV99 Tt Nanos Tt Wnt10
BV104 Tt Nanos Tt Wnt16

Debug PCR for Wnt contamination

The PCR shows that the actual Wnts were being cloned. LB medium used for transforming or growing should be the culprit.