Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 7 in /home/customer/www/phd.organelas.com/public_html/wp-content/themes/p2/inc/mentions.php on line 77

Relaxation with MgCl2: worked fine only about 2 minutes for immersed juveniles stop moving, although some were not completely stretched (maybe because they can’t). Recovering from it took a little longer, but they were reacting every water change (about 4 times were sufficient, around 10 minutes).

Fixation for in situ: Tried with a whole egg mass ripped to expose the egg bundles. It seemed ok (fixation and washes in Ptw) until the addition of Methanol when, apparently all the egg bundles became immersed in a jelly, maybe the inner linings dissolved with the Ptw and were condensed with the methanol? Tomorrow I’ll see how easy it is to dissect and if the eggs were not damaged.