mRNA extraction for Lineus ruber 

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Selected 1 egg mass for each time-span, dissected the bundles out, without disrupting the membrane, and kept them in gelatin coated dishes overnight. Egg bundles (and juveniles) were transfered to another dish and water exchanged once to get rid of dirt and ciliates. All bundles were put into a proper RNA eppendorf for the extraction DynaBeads protocol (link).

Samples: 2, 4-5, 8, 11, 16 days.

mRNA concentration: 23.8 ng/┬ÁL

mRNA Lineus ruber