More Membranipora genes: Mex3, Fez, PL10, FoxG, Syt1a, Syt1b

Set a PCR to clone: Mex3, Fez, PL10, FoxG, Syt1a, Syt1b

02/04/12

Bands with expected lengths. Purified DNA, set ligation reaction for 1h45 at RT, heatshock transformation and plated colonies with Xgal.

03/04/12

Plates were wetty, so colonies did not spread well… some were forming strings; but enough to pick. Picked white colonies for colony PCR:

09/04/12

Sequencing PCR and dropped in the SeqLab.

BV75 Membranipora membranacea Mex3 T7
BV76 Membranipora membranacea Fez T7
BV77 Membranipora membranacea Fez T7
BV78 Membranipora membranacea Fez T7
BV79 Membranipora membranacea PL10 T7
BV80 Membranipora membranacea PL10 T7
BV81 Membranipora membranacea PL10 T7
BV82 Membranipora membranacea FoxG T7
BV83 Membranipora membranacea FoxG T7
BV84 Membranipora membranacea FoxG T7
BV85 Membranipora membranacea Syt1a T7
BV86 Membranipora membranacea Syt1a T7
BV87 Membranipora membranacea Syt1a T7
BV88 Membranipora membranacea Syt1b T7
BV89 Membranipora membranacea Syt1b T7
BV90 Membranipora membranacea Syt1b T7