In situ Membranipora nanos, piwi2, maelstrom, pumilio, tudor

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Treated the samples for 1 min in proteinase-k, and then 2 washes in glycine. Put in prehybe at 20:00.


Started hybridization at 14:00.


Regular washes.


Developing from 11:30.


In the last days there were no embryos left (only some cyphonautes shells). The 1 min prot-k treatment with regular 5 min washes in glycine seems to be too much for them. Also, the longer period in prehybe possibly was not nice either.