In situ Membranipora nanos, piwi2, maelstrom, pumilio, tudor 

Treated the samples for 1 min in proteinase-k, and then 2 washes in glycine. Put in prehybe at 20:00.

25/03/12

Started hybridization at 14:00.

27/03/12

Regular washes.

29/03/12

Developing from 11:30.

Summary

In the last days there were no embryos left (only some cyphonautes shells). The 1 min prot-k treatment with regular 5 min washes in glycine seems to be too much for them. Also, the longer period in prehybe possibly was not nice either.