Probe PCR for Membranipora NANOS, PIWIL2, MAEL F2, PUM, TDRD1


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Clones amplified for riboprobe synthesis:

Gene ID Probe Kit
NANOS BV3 SP6
PIWIL2 BV6 SP6
MAEL BV9 SP6
PUM BV21 T7
TDRD1 BV23 T7

Probe PCR: 12:00. DNA extraction and purification: 18:30.

20/01/12

Agarose gel was ok, products had the expected length.

Probe Mm 2012-01-20 10hr 20min

Quantification of PCR product was also ok:

Sample ng/┬Ál 260/280 260/230
NANOS 574.73 1.91 1.92
PIWIL2 480.20 1.94 1.35
MAEL 300.42 2.05 0.56
PUM 293.22 2.00 0.53
TDRD1 224.36 2.00 0.48

Mm ProbePCR 20.01.12

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