Extra heat shock transformation

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I did an additional heat shock transformation using new Aina’s newest cells and the most recent ligation. Used the following samples Bn PUM1 F2, Mm MAEL F2, Mm PUM, Mm TDRD1, Control 1 (1.5 ┬Ál of ligation mix, what was left) and plated at 17:00.


Not enough colonies.