MiniPrep for Membranipora PIWIL2 and NANOS

09/01/12

Two positive colonies form Mm PIWIL2 (1 and 3) and Mm NANOS (2 and 3) were picked and added to a growth tube with 3 mL of LB medium. Tubes were left in the S8 large shaker overnight at 210 rpm / 37 °C.

10/01/12

1.5 mL were poured to eppendorfs, centrifuged, discarded supernatant and followed the MiniPrep protocol. DNA was kept in the fridge overnight before the sequencing reaction.

11/01/12

PCR sequencing reaction and submitted samples:

ID Gene Primer Quality Probe Kit
BV1 PIWIL2 T7 bad
BV2 PIWIL2 T7 bad
BV3 NANOS T7 ok SP6
BV4 NANOS T7 ok SP6
BV5 PIWIL2 SP6 bad
BV6 PIWIL2 SP6 short, ok SP6

13/01/12

Sequences came back, see above.