Colony testing for Mm PIWIL2, NANOS, PUM, TDRD1, and Bn PUM1, DDX4

Colony picking and testing for the following genes from the RACE PCR and regular PCR with Membranipora genes. PCR started at 14:20 and samples consist of:

Gene Colonies
Bn PUM1 F2 1
Mm DDX4 R2 1
Mm PIWIL2 8
Mm NANOS 8
Mm PUM 5
Mm TUDOR 3

Mm PIWIL2 and Mm NANOS had positive colonies. Mm PUM, Mm TDRD1, and Bn PUM1 F2 were negative. Mm DDX4 R2 is probably not positive (less than 500bp).

Colony check 2012-01-07 18hr 21min