PCR product check

Since no colonies grew in the first try of the RACE PCR repeating Bugula genes and new Membranipora genes, I ran a product check with 2 µl of sample + 2 µl of loading dye; also including the repetition of the regular PCR from Membranipora. Products seem to be ok although Mm DDX4 R2 and Mm PIWIL1 R1 had very faint bands. Which strongly suggests, as before, that the problem lies on the ligation/transformation steps.

PCR check for RACE and regular PCRs