Membranipora PCR repetition 

Since the ligation was not working well I am repeating the PCR for PIWIL2, NANOS, PUM1, and TRDR1 — previously done here using the same settings. The only difference is that I set the elongation time to 2 min and not 4 min. Also, I will not add extra loading dye for running the gel, as I mistakenly did last time.

05/01/12

PCR started 11:00 and the bands looked ok in the gel. Mostly identical to the previous one:

Membranipora PCR for Piwi, Nanos, Pumilio, and Tudor

I extracted the samples and set up a ligation starting at 20:00 overnight at 4 °C.

06/01/12

Heat shock transformation and plating at 18:30.

07/01/12

All plates had colonies as shown below:

Gene Colonies
PIWIL2 16
NANOS 10
PUM 5
TDRD1 3

PCR for colony testing started at 14:20.