Summary 2011 Bugula/Membranipora germline quest

PCR products look ok for all genes except for Bn PIWIL1 R2; there was no band.

Bugula neritina

  • Repeat RACE PCR for Bn PIWIL1 R2 and Bn PUM1 R2.
  • Repeat ligation and transformation for Bn PUM1 F2.
  • Isolate more germline genes via BLAST.

Membranipora membranacea

  • Repeat ligation and transformation for Mm PIWIL2, Mm PUM, and Mm TUDOR.
  • Do RACE PCR for the remaining genes (Mm PIWIL1, Mm DDX4, and Mm MAEL).
  • Prepare Mm NANOS for identity check with sequencing.