First outline 

Introduction

  • Quick historic review with 19th century comparative embryology and the buzz around Modern Synthesis’ lack of development concepts, with emphasis on marine invertebrate negligence.
  • Who are the marine invertebrates and why are they (and their development) so special.
  • The return of development and potential of recent evodevo research.
  • Quick note on Brazilian lack of developmental biology research and unknown marine biodiversity.

Proposal

  • Main goal with the project: comparative embryology of unstudied marine invertebrates through modern microscopy and molecular techniques directed to evolutionary questions.
  • Generic examples on how to achieve that detailing approaches and methods, as well as potential taxa, with a few examples.

Research example

  • Focused on bryozoans. Short intro about what they are and phylogenetic position.
  • Embryonic and larval developmental patterns to introduce main questions of the project.
  • What is the embryonic origin of larval structures? Cell lineage tracing to map the fate and elucidate the processes that form the larval parts, specially the fate of internalized cells during gastrulation (mesoderm origins?).
  • Development of the adult body during metamorphosis. How (and from which tissues) adult structures develop. Goes a bit into identity of blastemal cells.
  • Both above can be put in a comparative framework, using different species and types of larvae (cyphonautes and coronates) to provide some insight into the observed developmental patterns.
  • Finally, compare the development of the polypide during ancestrula formation and during polypide replacement using gene expression patterns.
  • Closure explaining the interestingness of these data for understanding developmental and evolutionary processes.